Why are you asked to gram stain a mixture of gram positive and gram negative bacteria on the same slide?

Answers

Answer 1
The accuracy of the Gram stain is dependent on the integrity of the bacterial cell wall. Variety of things can influence the cell wall integrity. Suppose the cells have been roughly handled , gram positive cells will come out as gram-negative. If you de-colorize them for too long, Gram-positive cells will look like Gram-negative cells or if you do not decolorize enough, Gram-negatives will look like Gram-positives. Under these circumstances Gram-positive and a known Gram-negative on the same slide so that the results are reliable.
Answer 2
Final answer:

Gram staining is a technique used to differentiate and classify bacteria based on their cell wall structure. By gram staining a mixture of Gram-positive and Gram-negative bacteria on the same slide, you can compare and contrast their staining reactions and overall characteristics.

Explanation:

Gram staining is a technique used to differentiate and classify bacteria based on their cell wall structure. Gram-positive bacteria have a thicker peptidoglycan layer in their cell wall, while Gram-negative bacteria have a thinner peptidoglycan layer. The differences in cell wall composition allow for differential staining during the Gram staining process. Gram-positive bacteria retain the purple crystal-violet stain, appearing purple, while Gram-negative bacteria lose the crystal-violet stain and instead take up the red safranin counterstain, appearing pink.

In the case of a mixture of Gram-positive and Gram-negative bacteria, gram staining on the same slide can help determine the Gram stain reaction, as well as the cellular morphology and arrangement of both types of bacteria. By observing both types of bacteria in a mixed smear, it becomes easier to compare and contrast their staining reactions and overall characteristics.


Related Questions

Explain why the formation of abscission layer causes sugar to accumulate in leaves.

Answers

Abscission layer is the layer of cutinized parenchyma cells that develops in the abscission zone. The abscission zone form across the base of the plant part, where the break eventually occurs. During the photosyntesis chlorophyll using sunlight produces sugar and storches which are used as food by plant. And sugar accumulate in leaves.   

How do you think sub-atomic particles (protons, neutrons, and electrons) affect the atom and its behavior?

Answers

Protons and Neutrons affect the mass. Protons and Electrons change the charge of the atom (positively or negatively charged). Valence electrons are the ones that do fun stuff in chemical reactions, they are responsible for making/breaking bonds, at least to my understanding.

Hope this helps.

Final answer:

Subatomic particles such as protons, neutrons, and electrons define an atom's mass, charge, and identity. Protons and neutrons make up the atom's nucleus, with protons determining the element, while electrons orbit the nucleus, influencing how atoms interact and the properties of matter. Neutrons add stability and mass, affecting isotopes and certain behaviors.

Explanation:

To understand how subatomic particles such as protons, neutrons, and electrons affect an atom and its behavior, we must look at their roles within the atom. Protons, which have a positive charge, and neutrons, which are neutral, comprise the nucleus of an atom. This nucleus gives mass to an atom, and the number of protons specifically determines the element's identity. On the other hand, electrons are negatively charged particles that orbit the nucleus, and their number generally equals the number of protons, balancing the charge of an atom.

The behavior of electrons around the nucleus is not random but follows principles of quantum mechanics, which suggests an ordered arrangement in shells and subshells. These arrangements determine how atoms will interact with each other, leading to the formation of molecules and, subsequently, the properties of matter.

Since neutrons do not have a charge, they primarily contribute to the mass and stability of the nucleus rather than interacting with the electrons or other charged particles. However, the number of neutrons can affect the isotope of an element, which in turn can impact the atom's behavior in certain situations.

Which two characteristics describe reputable digital resources

Answers

Is there answer choices you can show us?

Usually a reputable resource will be found on an informational or scientific site (especially something that is .edu or .gov) and will have a date written and author credited.

Answer:

Reputable digital resources must be

a) accurate

b) Authorized

Explanation:

Two characteristics which describe reputable digital resources are  

1. A reputable digital resources must be accurate – The information provided by a digital resources must be reliable and free from error else its authenticity becomes questionable.  

2. A reputable digital resources must have a authorship of its website. Thus all the information provided on the website must be referenced.  A good web source must have a contact production person.

The _____ lobe is important in processing visual information.

Answers

The occipital lobe is important in processing visual information. Hope this helps!

Answer:

The given blank can be filled with occipital lobe.

Explanation:

At the posterior part of the brain occipital lobe is situated that plays an important role in the interpretation of the visual stimuli and info. The primary visual cortex is situated in the occipital lobe that interprets and attains data from the retinas of the eyes. Destruction to this lobe may result in visual issues like the incapability to determine colors, difficulty in identifying objects, and others.

What is true of the bottom layer of the ocean? Select the two correct answers.

A. It has the most light.
B. It has the highest temperature.
C. It has the least salt.
D. It has the fewest plants.
E. It has the highest density.

Answers

Answer:

D. It has the fewest plants.

E. It has the highest density.

Explanation:

Since the water in the bottom of the sea has the highest density, and the lowest light, this layer is called the hadalpelagic zone, and it goes from 6,000 meters or 19,686 feet, up untill the lowest point known in the ocean that is located in the mariana trench, the temperature is found to be just above the freezing point and the pressure is eight tones per square inch, this makes it the most dense water in the ocean and it does not allow plants to grow properly, invertebrates thrive on those conditions.

A baby is born with ambiguous genitalia. what should the nurse emphasize when discussing this with the parents

Answers

She should first emphasize the that it's more common than we think and this is not parent's fault (sometime, most of them feel guilty about that). The chirurgical surgery can be done at any age and without many risks, it's a common surgery with great results. The main problem is the choice the parents have to do : wait and let the child decide, do the surgery to get a boy, do the surgery to get a girl. She can adds that a decision is not required now and they can let the baby grow up a little bit to decided. Kids may have psychological sequelae or questions about her/his sexual identity especially at adolescence. Psychological follow-up may be envisaged and, in all cases, upholding from parents is essential.

Chemical energy is a form of _____ energy. chemical energy is a form of _____ energy. entropic potential heat motion kinetic

Answers

The answer is potential. It is because chemical energy is a storage of energy that are formed in which making it or why it is considered to be potential rather than those given choices above in which doesn’t describe chemical energy.

Chemical energy is a form of potential energy. The correct option is B.

Potential energy takes the form of chemical energy. It is kept in the bonds that bind molecules and atoms together.

The chemical energy that has been stored is released during a chemical reaction. Chemical energy is used all around the world and is essential for many operations.

Batteries, biomass, oil, natural gas, and coal are a few examples of chemical energy sources. When substances go through chemical reactions, other types of energy, such thermal energy, can be created.

Thus, consequently, the appropriate response is B. Potential energy.

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Your question seems incomplete, the probable complete question is:

Chemical energy is a form of _____ energy.

A. entropic

B. potential

C. heat

D. motion

E. kinetic

scientific observations: a. are limited to available equipment used to record a natural phenomenon. b. can be made by instrumentation c. can be made directly by humans d. all of the above e. none of the above

Answers

d) all of the above are correct

Jane is performing an experiment on plant growth. She places the plant in direct sunlight, waters it the same time every day, and always gives the plant the same amount of water. What is the dependent varible

Answers

Jane is measuring plant growth, so this would be the dependent variable.
The same time of day that she waters the plant and the same amount of water would both be examples of controls. 

ll theories are hypotheses, but not all hypotheses are theories. Francis wants to know if a specific hypothesis he is researching is a theory. Which describes how Francis would know the hypothesis is a theory? He could restate the hypothesis as a fact and no longer an explanation. He could determine whether the hypothesis is based on repeated experiments. He could perform the experiment and adjust data to match the desired results. He could design and perform a new controlled experiment to test the hypothesis.

Answers

The correct option is this: HE COULD DETERMINE WHETHER THE HYPOTHESIS IS BASED ON REPEATED EXPERIMENTS.
An hypothesis is a proposed explanation based on prior knowledge which serves as a starting point for scientific experimentation. After the experiment, if the hypothesis is confirm to be true and other scientists test it also and find it to be true, then the hypothesis can be regarded as theory, because it has been tested many time and find to be true.

Answer:

b

Explanation:

What are the chemical differences between the cell walls of gram positive and gram negative bacteria that might explain differences in the rate of decolorization?

Answers

The cell wall is the layer that lies outside the plasma membrane. The chemical difference between the cell walls of gram positive and gram negative bacteria are as follows-

Gram positive bacteria

The cell wall of gram positive bacteria is thick as compared to the gram negative bacteria. It consist of a single 20-80nm thick peptidoglycan layer.

Gram negative bacteria

In gram negative bacteria the cell wall comprises of peptidoglycan layer and outer membrane. The peptidoglycan layer is 2 to 7 nm thick and it is covered by a 7 to 8 nm thick outer membrane.

During staining procedure the bacteria are first stained with crystal violet and then treated with the iodine to promote dye retention. After this  When gram positive bacteria are treated with alcohol (ethanol) for decolorization, the alcohol shrinks the pores of the thick peptidoglycan cell wall, which retains the crystal violet dye-iodine complex and the gram positive bacteria remains purple. However, the peptidoglycan layer of gram-negative bacteria is thin and has large pores because of which alcohol easily removes the crystal violet dye-iodine complex from the cell wall and gets easily counterstain  by safranin.

So, the rate of decolorization in gram negative bacteria is faster than the rate of decolorization in gram positive bacteria due to the presence of thin peptidoglycan layer in gram negative bacteria.

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Final answer:

The cell walls of gram positive bacteria contain thick layers of peptidoglycan and teichoic acids, retaining crystal violet dye, whereas gram negative bacteria have a thin peptidoglycan layer and an outer membrane with LPS, decolorizing faster and taking up safranin dye.

Explanation:

The major chemical differences between the cell walls of gram positive and gram negative bacteria involve the structure and composition of their cell walls, which impacts the rate of decolorization. Gram-positive bacteria have thick layers of peptidoglycan, which retain the crystal violet dye during the Gram staining procedure, making them appear purple. They also contain teichoic acids, which gram-negative bacteria lack. Conversely, gram-negative bacteria have a much thinner layer of peptidoglycan and possess an outer membrane that contains lipopolysaccharides (LPS). This outer membrane dissolves during the alcohol wash in the staining process, allowing the crystal violet dye to escape. Afterwards, these cells take up the pink dye safranin and appear pink. The presence of LPS in the outer membrane of gram-negative bacteria also gives them the ability to release endotoxins.

Which question most closely relates to the foresters observations and the collected scientific data on forests?

A. Did a lack of Rain cause the hemlocks to stop reproducing?

B. Are deer eating most of the young hemlocks?

C. Do the young hemlocks have a disease?

D. Why are there so many deer in the forest?

Answers

The answer is letter b, are the deer eating most of the young hemlocks? Hemlocks are a common type of tree in the northern forests of the United States. We know that deer will eat young hemlocks but not old hemlocks because they are still growing and have more nutrition.  If the deers are eating young hemlocks, then keeping the deers away from the young trees will allow the trees to grow. 

Correct answer choice is :


B) Are deer eating most of the young hemlocks?Explanation:

A forester is a person who studies forestry, the science, art, and business of maintaining forests. Foresters interest in a wide range of activities including environmental rehabilitation and management of preserved areas. Foresters are included in a large range of exercises covering environmental restoration, timber gathering, and day-to-day administration of protected areas.


Which type of energy is provided by protein macromolecules?

Answers

Carbohydrate is the major source of energy in the body, but protein and fat too can be used for energy production in time of emergency. When a system is deprived of energy, the body can convert protein to carbohydrate in form of glucose in order to provide the necessary energy. Thus, protein is used to provide emergency energy.

Based on what you know about the relative temperatures of Earth’s geological layers, what can be said about the crust, mantle, and core of other terrestrial planets? For any terrestrial planet, the would be the coolest layer, and the would be the hottest layer.

Answers

The crust would be the coolest and the core would be the hottest . Hope this helps:)

Answer:

Any terrestrial planet has highest temperature at its core and least temperature at its crust.  

Explanation:

Considering the composition of earth, there are three main layers whose composition and temperature is given as follows –  

a) Inner Core – The inner core is made up of iron and nickel and has a temperature of 7200 degree Celsius.

b) Molten Outer core – It is also made up of iron and nickel and has a temperature of 4300 degree Celsius.

c) Inner mantle – It is semi rigid and made up of silicon, oxygen, magnesium, iron, aluminum, and calcium and has a temperature of 3700 degree Celsius

d) Crust – It is made up of water, basalt and granite and has a temperature of 0 degree Celsius

Thus, looking at the composition and temperature of different layers of earth , it can be said that any terrestrial planet has highest temperature at its core and least temperature at its crust.  

Elliptical galaxies are egg shaped galaxies without spiral arms or interstellar gas and dust. True or false

Answers

It would have to be true.

Answer,

True

Hope This Helps :-)

Each level of the energy pyramid captures about _______ percent of the energy found in the level below it.

Answers

Ten. The other ninety percent of energy that is used by the organism is just to stay alive. This is because cellular processes require a lot of energy. Think about all the protein that has to be synthesized just for translation. Maintaining the cell and having resources to increase in cell division just for the organism to grow.

You would most likely find in a volcano.

Answers

You would most likely find Archaea in a volcano. Archaea is one o the oldest kind of single-cell organism which could survive an environment with a really high temperature, which is why you could see no organism beside Archaea in a Volcano and nothing else.

Why is baker's yeast useful for expressing genetically engineered genes?

Answers

Yeast cells are eukaryotic and so would likely be successful in expressing eukaryotic genes.

Baker's yeast is useful for expressing genetically engineered genes as yeast cells are eukaryotic and so would likely be successful in expressing eukaryotic genes.

What are the characteristics of yeast cells?

Yeast cells are round to long cells that reproduce vegetatively by budding or germinate to produce a mycelium. They are found in some members of the Mucorales.

The yeast cell wall is a dynamic organelle that determines the cell shape and integrity of the organism during growth and cell division. It must provide the cell with mechanical strength in order to withstand changes in osmotic pressure.

An important feature of these yeasts that makes them such useful organisms for studying biological processes in humans, is that their cells, like ours, have a nucleus containing DNA, packaged into chromosomes. Most metabolic and cellular pathways thought to occur in humans, can be studied in yeast.

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The condition of the blood and the amount of ____________ can influence skin tone.

Answers

The amount of blood flow can influence the skin tone of a person. The blood enters the heart through two large veins, which called the superior and inferior vena cava, emptying oxygen-poor blood from the body into the right atrium. Hemodynamics is the term called for the study of blood flow.


Genes that are linked together _____.

combine to form traits

result in incomplete chromosomes

form dominant traits

are found on a particular chromosome

Answers

Final answer:

Linked genes are found on the same chromosome and are inherited together, affecting the inheritance patterns of characteristics. Geneticists use recombinant gametes to map gene positions and distances on chromosomes.

Explanation:

Genes that are linked together are found on a particular chromosome. These linked genes tend to be transmitted through meiosis together, resulting in certain characteristics frequently inheriting together. For instance, linked genes are responsible for the co-occurrence of certain hair and eye colors. The position of two linked genes on a chromosome affects the likelihood of crossovers during meiosis, with closer genes being less likely to separate than those that are farther apart.

Linkage maps created by geneticists utilize the proportion of recombinant gametes to determine the distance between genes on a chromosome. This information helps to understand genetic inheritance patterns and the probability of recombinant offspring in genetic crosses that do not follow the classical Mendelian dihybrid 9:3:3:1 ratio.

The population of frogs at a lake is 12 frogs per square meter. What will be the estimated population of frogs in a habitat of 370 square meters? The estimated population of the frogs will be ______.

Answers

4440 frogs
not including variables.

List 3 variables that may determine the choice of which dc polarity to use

Answers

The direction of the flow of electrical current,weather or not the transmitter allows current to flow through simultaneous output and if the capacity or load to take the current is grounded.

When auscultating an animal's lungs, you separate the right and left thorax into _______ quadrants.
a. seven
b. nine
c. five
d. three?

Answers

The answer to this question would be nine quadrants

Auscultating is an examination where you put stethoscope into the patient chest and try to hear their breathing/heart sound. The lung is a big organ but contains multiple lobes, so when there is an abnormality in a part the sound might not be heard in the other lobes.
It is important to auscultate the lung into at least 9 quadrants so you won't miss it.

How does the cylindrical shape of the palisade mesophyll cells increase the amount of photosynthesis that the leaf can cary out?

Answers

Answer:

The cylindrical shape allows more cells to be packed into space, which allows for more chloroplasts, and more photosynthesis to occur.

Explanation:

my teacher approved my answer.

The cylindrical shape of the palisade mesophyll cells increase the amount of photosynthesis that the leaf can carry out the cylindrical shape allows more cells to be packed into space, which allows for more chloroplasts, and more photosynthesis to occur.

What is photosynthesis?

The process of photosynthesis has been defined as the the process in which green plants prepare their own food in the presence of sunlight and chlorophyll present in plants helps in this process. During this process heat energy converted into chemical energy.

Tissues has been combined together and they form the muscles and these muscles are of several types and after that the muscles combined together and they form organ and each of the organ of the body is made up of different types of tissue. Like heart is made up of such type of tissue that never takes rest.

Therefore, The cylindrical shape of the palisade mesophyll cells increase the amount of photosynthesis that the leaf can carry out the cylindrical shape allows more cells to be packed into space, which allows for more chloroplasts, and more photosynthesis to occur.

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The relationship between the skin and the muscles can be described as

Answers

The skin is superficial to the muscle and the muscle is deep to the skin

This best describes the relationship between the skin and the muscles. The skin is superficial when it comes to muscle function and operation. And, the muscle is deep to the skin. It is below the skin, not attached to the skin, and does not serve to aid the skin in basic functions.
Final answer:

The skin and muscles have an interconnected and interdependent relationship, with the skin protecting the muscles and the muscles providing support and movement to the skin.

Explanation:

The relationship between the skin and the muscles can be described as interconnected and interdependent. The skin, which is the largest organ in the body, is responsible for protecting the muscles and underlying structures. It also plays a role in regulating body temperature and sensation. In turn, the muscles provide support and movement to the skin, allowing for actions such as facial expressions and locomotion.

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What hypothesis were scientist trying to prove by conducting the Fox Farm Experiment?

Answers

The scientist, Dimitry K. Belayev, was trying to prove that dogs's physical traits- and morphological traits- were changed by natural selection. He thought that the dogs that were friendliest towards humans were selected, and that changed them. To prove it, he domesticated an animal which is very similar to a dog, a fox.
Final answer:

The Fox Farm Experiment was designed to test a hypothesis about genetic selection and domestication, involving the selective breeding of foxes to study changes in their behavior and physical traits. This research approach is essential for testing the validity of scientific predictions and expanding our understanding of underlying biological processes.

Explanation:

Scientists conducting the Fox Farm Experiment were trying to prove a hypothesis, which is a proposed explanation that attempts to interpret an observation or answer a specific question about the natural world. The hypothesis in question was likely related to genetic selection and domestication, as the famous Fox Farm Experiment by Dmitri Belyaev involved selecting foxes based on their tameness to study the process of domestication and its effect on physical and behavioral traits. An experiment like this typically serves as a means to test predictions derived from a hypothesis, allowing for rigorous scientific scrutiny and the potential to refine or refute the initial proposal based on empirical evidence.

To test the hypothesis, researchers would have set up controlled experiments comparing different groups of foxes or other variables, much like comparing the number of deformed frogs in ponds with different levels of a certain chemical. A well-constructed hypothesis not only addresses the initial observation but also provides a basis for making further predictions that can be empirically validated through experimentation.

Describe the skin color of someone who is in the first stages of hypothermia

Answers

Typically the skin will be cool and dry, except in cases of immersion in fluids, with mild hypothermia. Exposed skin may begin to turn purple and puffy once core body temperature falls below 90 degrees Fahrenheit. The patient will typically shiver if they have mild hypothermia only ceasing to do so after their core body temperature has fallen below 93 degrees Fahrenheit.

The bacteria Vibrio vulnificus is sometimes released into the ocean by sewage pollution. This small bacterium is often eaten by filter feeders, such as oysters. What impact would you expect this to have on individuals that consumed oysters which had fed on the bacteria?

A.) The bacteria would have a negative impact on the oysters but is harmless to humans.
B.) The bacteria could cause infections in people who consume contaminated shellfish.
C.) The bacteria could cause infections in people who do not have strong enough immune systems to fight off the bacteria, which could then be transmitted person to person.
D.) The bacteria would not cause infections in any people because our immune systems are strong enough to fight off the bacteria.

Answers

The answer would be B

Answer:

B.) The bacteria could cause infections in people who consume contaminated shellfish.

Explanation:

The bacteria Vibrio vulnificus could cause infections in people who consume contaminated shellfish. Individuals with certain medical problems and weak immune system exhibit severe infections. Consumption of cooked shellfish is recommended to reduce the risk factors of bacterial infection. The  bacteria Vibrio vulnificus cannot spread from one infection person to other healthy person.  

A pattern has 5 blue triangles to every 80 yellow triangles.what is the ratio of blue triangles to all triangles

Answers

In order to determine the ratio, we simply make the fraction:

Number of blue triangles / number of yellow triangles

= 5/80

Next, we simplify the numerator and denominator using the highest common factor, which is 5.

= 1/16

Therefore, the ratio of blue triangles to yellow triangles is 1 to 16.

The caregiver of a 4-year-old girl who lives in a heavily wooded area calls the clinic nurse to report that the child has a swollen tick on her arm. what would be the best action for the caregiver to follow in removing the tick?

Answers

Taking the child to a doctor for treatment for the tick bite so the child doesnt get sick.
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Perhaps you wanted pizza for dinner, but were out voted by the rest of the family who wanted chili. This is similar to what happens in a community. One person has to give up a right for the good of the group. Sometimes citizens' duties and rights conflict with each other. A good example is a public protest. People have the right to meet in groups and share ideas. However, a protest can disrupt traffic or other normal activities. A city must provide extra police protection to keep people safe. Therefore, the city has the right to require permission in advance for a protest. Government must make laws to balance the rights of individuals and different groups of people. What type of connection is used in the paragraph? (5 points) Simile Contrast Category Analogy which aspect of the following poem might imagists most admire? A(an) ________ is the best example, or representation, of a concept. In recent years, the supreme court has trended toward empowering _____ level(s) of government. Process in which cells develop special structures and functions Describe two push factors involved in a current refugee crises?Plz i need help Which of the following is a subordinate adverb? Select all that apply.a:ifb:afterc:therefored:whenever 12. What is most likely result of increased labor productivity?A. Producers will raise the prices for their goodsB. Producers will make goods more quicklyC. Producers will hire many more workersD. Producers will experience higher demand for their goods13. What is the main purpose of a market?A. to allow people to exchange goods and servicesB. to set prices for goods and services C. to help producers get human resourcesD. to increase competition between buyers and sellers Michael made 4 dozen chocolate chip cookies he is bringing 75% of them to school for a party how many cookies does he bring to chool To put it in the terms of st. Thomas aquinas: an unjust law is a human law that is not rooted in eternal law and natural law. Any law that uplifts human personality is just. Why does king use this allusion The ______ Basin contains the world's largest expanse of tropical rain forest. A. Amazon B. Rio Negro C. Salimes D. Orinoco What happened after President Clinton sent peacekeeping troops to Somalia in 1993? 35.7 divided by 11.4 The nurse admits an infant who is nutritionally deprived. the infant is weak and seems somewhat uninterested in food. in developing the infant's plan of care, how often will the nurse most likely plan to feed this infant? Help Needed xDWhat is the value of X? Remember to show calculations and formulas used for credit.Question 1 options:SaveQuestion 2 (3 points)What is the measure of one of theexterior angles of a regular octagon?Make sure to show your calculation and formulas used for credit.Question 2 options: Alicia is going to college and working a full-time job. Her employer generally supports her decision to be in college, but Alicia thinks her boss is asking her to take on more responsibilities than her schedule allows. Alicia decides to arrange a meeting in which she hopes they can both talk frankly about the problem, but she worries that her boss will think shes being a complainer. 1 of 3 What should Alicia do to prepare for her meeting with her boss? A. Draft a resignation letter to give her boss at the start of the meeting in case they cant come to an agreement. B. Plan to discuss the ways attending college is helping Alicia be better at her job. C. Nothing. Conversations like this are always best when they are spur of the moment and dont seem staged. D. Tell other coworkers and HR that she suspects her boss is deliberately giving her more work than she can reasonably handle while also attending school in an effort to get her to withdraw from her coursework. Evaluate 3x+y3x+y when x=13x=13 and y=74y=74. write your answer as a fractionin simplest form. In which stage or stages of the business cycle would there be more personal bankruptcies more business formation? Why is erythropoietin secreted in larger quantities in patients with anemia or anoxic stress? Calculate the molarity of 0.850 mol of Na2S in 1.70 L of solution. Steam Workshop Downloader