What kind of pollution was the most abundant in the US national parks in 1989? How did this pollution affect park wildlife and ecosystems?
Describe how a plasmid can be genetically modified to include a piece of foreign dna that alters the phenotype of bacterial cells transformed with the modified plasmid. describe a procedure to determine which bacterial cells have been successfully transformed.
Final answer:
A plasmid can be genetically modified to carry foreign DNA using recombinant DNA technology. Bacterial transformation is the process where bacteria take up the modified plasmid, which can then be replicated within the bacteria. Selective growth in antibiotics allows for the identification of successfully transformed cells.
Explanation:
Genetic Modification of Plasmids and Bacterial Transformation
To genetically modify a plasmid to include a piece of foreign DNA that alters the phenotype of bacterial cells, recombinant DNA technology is used. A plasmid is a circular DNA molecule that acts as a vehicle for genetic material. Scientists insert the foreign DNA into the plasmid using restriction enzymes and DNA ligase, creating a recombinant plasmid. This plasmid can then be introduced into bacterial cells through a process called transformation, where bacteria take up free DNA from their surroundings. In the lab, bacterial cells are made more permeable to DNA, often through chemical treatment or electroporation to facilitate the uptake of the recombinant plasmid.
Once inside the bacterial cell, the recombinant plasmid is replicated along with the bacterium's own DNA. If the plasmid contains a gene that confers antibiotic resistance, transformed cells can be selected by growing them on media containing the antibiotic. Only bacteria that have successfully taken up and expressed the antibiotic resistance gene will survive. Other markers, like the gene for green fluorescent protein (GFP), can also indicate successful transformation if the bacteria exhibit fluorescence under specific lighting conditions.
Finally, to answer what would happen if we use a different antibiotic like kanamycin instead of ampicillin, bacteria would need to carry a plasmid with a gene that provides resistance to kanamycin. Without such a gene, the bacteria would not grow in the presence of kanamycin.
The term mixotroph indicates that a protist __________.
What characteristic of agar makes it a useful solidifying agent for growth media? select one:
a. it can be completely liquefied at 42°c?
What type of questions cannot be solved by scientific method?
A. Questions about ethics
B. Questions about extinction species
C. Questions about the deep ocean
D. Questions about the past
The correct answer is option A
Scientific method is used to answer the question that is based on science. These questions can be answered by the help of various set of protocols which has to be followed one after another.
The protocols has to be followed in a proper manner such as aim, hypothesis, procedure, result and conclusion.
The questions related to ethics does not requires proper experiment. These questions can be answered based on person's opinion.
The scientific method cannot address questions about ethics, the past, or the future.
Explanation:The scientific method is a systematic approach used to answer questions through observation, experimentation, and analysis. While the scientific method is a powerful tool for investigating and understanding the natural world, there are certain types of questions that it may not be able to address:
Questions about ethics: Ethical questions involve subjective values and principles, which cannot be tested or measured using the scientific method.Questions about the past: While scientists can study the evidence available from the past (such as fossils or historical documents), they cannot directly observe or experiment on past events.Questions about the future: The scientific method relies on empirical evidence, which is obtained through observation and experimentation in the present. Predicting future events or outcomes may involve factors that are difficult to measure or control.In conclusion, the scientific method is a valuable approach for answering many types of questions, but it is not applicable to all areas of inquiry.
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A mother who is visiting the pediatric clinic with her 10-month-old son tells the nurse how pleased she is with her chubby infant. she exclaims, "look how much weight he's gained even though he drinks only orange juice! he won't drink any milk!" what is best response by the nurse?
Answer:
"He's a little overweight."
Explanation:
The baby may be slightly overweight. The nurse should tell this to the child's mother and indicate what steps to take in order for the baby to reach the ideal weight for his or her age.
In feeding the baby at 10 months parents should let the baby eat with their own hands. The baby wants to eat alone and takes all the food to his mouth with his fingers. Parents should let him eat alone and only in the end should they give what was left on the plate with the spoon.
The 10-month-old baby should also start eating more consistent, crumbly foods such as potatoes, peach or pear jam, mashed bread, bits of bread and not just orange juice.
Are you (an animal) more closely related to the mold in your shower (a fungus) or the oak tree outside your window (a plant)?
You (or an animal) are more closely related to fungi. This has been determined through molecular phylogeny analysis.
What is methanobrevibacter ruminantium's effect on digestion in cattle?
In Wisconsin, a very large population of lake trout, in which individuals mate at random, experiences no migration, mutations, nor selective pressure for a number of years. Which of these is most likely to occur in the population?
Fetal cells recovered during amniocentesis are grown in tissue culture. what mitotic phase are the chromosomes recovered from these cells arrested at for karyotype examination?
Women have sex chromosomes XX, and men have sex chromosomes XY. Which of a woman's grandparents could not be the source of any of the genes on either of her X chromosomes?
mother's father
father's mother
mother's mother
father's father
Answer:
Option). Father's father.
Explanation:
Humans are diploid organisms having 23 pairs of chromosomes (46 chromosomes). The 22 pairs of chromosomes are similar in both male and female, while the 23rd pair of chromosomes in male has one X and Y chromosomes and 23rd pair of chromosomes in female has two XX chromosomes.
During reproduction, a man provides Y chromosome to his son and X chromosome to his daughter, while female provides X chromosomes to son and daughter.
Hence, the father's father of a woman would provide only Y chromosome to his son (woman's father), which could not be a source of any X chromosome's gene of that woman.
Thus, the correct answer is option). father's father.
How can cyanobacteria, in contrast to saprotrophic bacteria, live in an environment that lacks organic nutrients?
Jill is planning on running the boston marathon and wants to avoid hyponatremia. what is hyponatremia
in 2008 a tornado swept across the northern part of Lake city, Florida damaging about 50 homes and a dozen business establishments. What would people in Lake city, Florida most likely do if a tornado alert is sounded?
A) they would rush to high lands
B) they would install heaters in their houses
C) they would find a safe room within a building
D) they would buy a tornado detecting instrument
Answer:
The correct answer is option C, that is, they would find a safe room within a building.
Explanation:
On the basis of the safety tips, which is generally released by the government, it is usually instructed that at the time of a tornado, the individuals should go in the interior room, that is, on the lowest bases of their houses, and the rooms should be without any window. It is also suggested that when the situation arises, one should get beneath a furniture and should use the arms and hands to protect the neck and the head.
Permafrost presently occurs in regions of eurasia and north america even where glaciers are not currently found
A client is very anxious and states "i am so stressed." how do stress and anxiety affect the client's safety and ability to retain information
You performed a blood typing activity as described in the lab manual. you observed agglutination (clumping) with anti-b and anti-rh serum. based on the test results you can conclude that the blood type you tested was ________.
Final answer:
The blood type tested is B-positive, as evidenced by the agglutination with anti-B serum and anti-Rh serum. A cross-match assay is a test to ensure blood transfusion compatibility, preventing potential reactions.
Explanation:
Based on the observation that there was agglutination with anti-B serum and anti-Rh serum, we can conclude that the blood type tested is B-positive. Agglutination occurs when antibodies bind to specific antigens on the surface of the red blood cells causing them to clump together. Since the anti-B serum caused agglutination, the blood contains B antigens. The presence of agglutination with anti-Rh serum also indicates that the Rh factor is present, which means the blood is Rh-positive (B+).
A/an __________ may involve anything from a minimal exchange of information to extensive contact with the birth mother before and after the child is born.
During dehydration synthesis, a ________ molecule is produced. A. water B. sodium chloride C. monosaccharide D. potassium chloride Mark for review (Will be highlighted on the review page)
In which one of the following locations can a person hear the echo of a sound?
Answer:
at least 16.6 meters of the reflecting surface
Explanation:
The network of glands that release chemical messengers directly into the bloodstream is called the ________.
Identify the start and stop codons in the mrna sequence 5'-uauccauggcacuuuaaac-3'. what is the resulting amino acid sequence
Answer;
-Start codon: AUG;
-Stop codon: UAA;
-Amino acid sequence: Met-Ala-Leu-stop
Explanation;
-A codon is a sequence of three DNA or RNA nucleotides that corresponds with a specific amino acid.
-The start codon marks the site at which translation into protein sequence begins, and the stop codon marks the site at which translation ends. The start codon is often preceded by a 5' untranslated region (5' UTR). AUG is the most common start codon. There are three RNA stop codons: UAG, UAA, and UGA.
It is on the genetic code that the biosynthesis of proteins rests. The DNA is transcribed into messenger RNA (mRNA). This is translated by the ribosomes that assemble the amino acids present on transfer RNAs (tRNAs).
The right answers are:
Start codon: 5'-uauccauggcacuuuaaac-3'. It codes for Methionine
Stop codon: 5'-uauccauggcacuuuaaac-3'
We will have three amino acids produced (each one has a codon of three nucleotides): 5'-uauccauggcacuuuaaac-3'.
Stop codon does not code for an aminoacid.
The AA sequence is Methionine-Alanine-Leucine.
A woman can read the large print in a newspaper only when it is at a distance of 67 cm or more from her eyes. (a) is she nearsighted (myopic) or farsighted (hyperopic), and what kind of lens is used in her glasses to correct her eyesight? (b) what should be the refractive power
(a) As women is able to see a distance of 67 cm or more from her eyes.
so, she has difficulty reading close objects.
So she is
farsighted (hyperopic)
To correct her eyesight converging lens or convex lens will be used.
(b) u = 24 cm -2.3 cm = 21.7 cm
v = -67+2.3= 64.7 cm
1/v+1/u =1/f
1/f =0.03063
f=32.651 cm
Power = 100/f = +3.0627
Whenever you turn the pages of a book, use your pen to take notes, or raise your hand, you know that this is possible due to your:?
Answer:
which means two things: I have a lot of books, and not a lot of anything else. So when people come over to my apartment and don’t want to talk to each other, they’re forced to marvel at my bookshelves, which are both my only art and my largest pieces of furniture.
After a couple of glasses of wine, I inevitably begin handing out my books like party favors. I always think I will get them back. But then I never get them back. And if I do, the pages are ripped, the cover clinging by one stitch, or there’s a hard, crusty peak of some unidentifiable food item (I hope) trapped on page 47.
Explanation:
The crystal of a mineral are always the same size. True or false
Type of active transport that helps muscle cells contract
Which of the following is an accurate description of mitosis and meiosis?
A. Both processes are always a form of sexual reproduction.
B. Both processes always change the number of chromosomes.
C. Mitosis produces two diploid daughter cells, while meiosis produces four haploid daughter cells.
D. Meiosis produces two haploid daughter cells, while mitosis produces four diploid daughter cells.
Answer: Option C Mitosis produxes two diploid daughter cells, while meiosis produces four haploid daughter cells.
Explanation:
Mitosis is a form of cell division which takes place to increase the cell number in the body.The process of mitosis consists of a single round of cell division such that a given cell divides to produce two daughter cells.During mitosis there is no change in the number of chromosomes of the cell thus, one cell divides and forms two diploid daughter cells. Hence, this division is also called as an equivalent division because it does not involve any change in chromosome number.Meiosis is a form of cell division which takes place to form the gametes during sexual reproduction.Meiosis takes place to divide a single cell into 4 cells.The reason that 4 cells are produced from a single cell is that there are two rounds of cell division which takes place in meiosis. Thus, a diploid cell divides in meiosis two times and forms four haploid cells.Thus, as the number of chromosomes are decreasing meiosis is known as reductional division.Besides race, what other things explain why some people might be more susceptible than others to disease? think about the girl in the film with sickle cell anemia. how is ancestry different from race?
The reason why some people might be more susceptible than others to disease is ancestry.
What is ancestry?Ancestry can be defined as a person's ethnic root, place of birth is a person, and the individuals parents.
What is race?Race refers to the grouping of people based on their physical and social qualities. People of the same race share the qualities, language and geographical location.
Therefore, ancestry is another reason why some people might be more susceptible than others to disease. People sharing the same ancestral lineage might be susceptible to some kind of diseases than others due to their weather or location.
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Ancestry, reflecting genetic lineage, influences disease susceptibility, unlike race, which is a socially constructed categorization based on physical characteristics.
Besides race, several factors contribute to variations in disease susceptibility among individuals, including genetic factors, environmental exposures, lifestyle choices, socioeconomic status, and access to healthcare. In the case of sickle cell anemia, ancestry plays a significant role in disease susceptibility.
Ancestry refers to a person's genetic heritage or lineage, often reflecting the geographic origin of their ancestors. In the context of sickle cell anemia, individuals with ancestry from regions where malaria is or was endemic, such as parts of sub-Saharan Africa, the Mediterranean, or the Middle East, may have a higher prevalence of the sickle cell trait. This genetic adaptation provides some protection against malaria but also increases the risk of sickle cell disease when inherited from both parents.
Ancestry differs from race in that it is based on genetic lineage rather than social or cultural constructs. Ancestry reflects biological relationships and genetic heritage, while race is a socially constructed categorization based on physical characteristics, cultural identity, and societal perceptions. Therefore, ancestry can provide important insights into genetic predispositions to certain diseases, such as sickle cell anemia, that transcend racial categories.
What are at least two major differences between eukaryotic and prokaryotic cells and one major similarity between the two?
Final answer:
Prokaryotic cells lack a nucleus and membrane-bound organelles, while eukaryotic cells have a defined nucleus and complex organelles. Yet, both share essential structures such as the plasma membrane, cytoplasm, nucleic acids, and ribosomes for basic cellular functions.
Explanation:
Differences between Prokaryotic and Eukaryotic Cells:
Prokaryotic and eukaryotic cells are distinguished by several critical characteristics. Two major differences between them are:
Nucleus: Prokaryotic cells lack a nucleus surrounded by a complex nuclear membrane and have a single, circular chromosome in a region called the nucleoid. In contrast, eukaryotic cells have a nucleus enclosed by a nuclear membrane containing multiple, rod-shaped chromosomes.Cellular structure complexity: Eukaryotic cells possess membrane-bound organelles, such as the endoplasmic reticulum and mitochondria, while prokaryotic cells do not have these structures, which translates into a more complex organization in eukaryotic cells.Major similarity: Despite these differences, both cell types share four common structures — the plasma membrane, cytoplasm, nucleic acids, and ribosomes, which are essential for various cell functions including protecting the cell, storing genetic information, and synthesizing proteins.
Could an elodea or paramecium from a freshwater lake be expected to survive if transported to the ocean? why or why not?